NMR analysis of budding yeast metabolomics: a rapid method for sample preparation.

نویسندگان

  • C Airoldi
  • F Tripodi
  • C Guzzi
  • R Nicastro
  • P Coccetti
چکیده

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by (1)H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

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عنوان ژورنال:
  • Molecular bioSystems

دوره 11 2  شماره 

صفحات  -

تاریخ انتشار 2015